Sentence examples for buffer to start the from inspiring English sources

750 μL from this cell suspension were added to 750 μL of substrate solution (4 mM in catalysis buffer) to start the biotransformation reaction.

After 5 minutes, the mycelia were transferred into fresh uptake buffer to start the reaction.

To start the experiment, buffer B (buffer A plus 0.1 mg ml−1 glucose oxidase, 20 µg ml−1 catalase, 5 mg ml−1 glucose plus 0.5% w/v methylcellulose, 400 cP) was flowed at 10 µl min−1.

Then 50  μL of 3.0 mM (pNPG) as a substrate dissolved in 20 mM phosphate buffer (pH 6.9) was then added to start the reaction.

First, the samples were subjected to a HiPrep 16/10 QFF anion exchange column (GE Healthcare, Taipei, Taiwan) preequilibrated with 60 mL of starting buffer to stabilize the UV signal, and then a 10-mL sample was applied and eluted for 60 mL with a step gradient using a 15% mixing ratio of elution buffer (50 mM Tris·HCl, 2 M NaCl, pH 7.0) at a constant flow of 3 mL/minute.

Long term, you can optimize this buffer to increase utilization rates, but to start, it is always best to err on the side of caution.

After starting the flowing buffer, a solution of 75 mM EDC and 15 mM NHS in water was injected into the flowing buffer to activate the carboxylic groups.

gradient to starting conditions (60% buffer A - 40% buffer B).

In the meantime, they head for the sand, the 100m buffer zone, and start to kick a ball around.

Touch the pad lightly to the surface before starting the buffer so the polish or compound doesn't spray in all directions.

When setting the start buffer to a pH of 5.5 or 6.0 the maximum breakthrough is 6% and 3% respectively.