Sentence examples for buffer to remove the from inspiring English sources

After the membranes were washed with TBS (Tris-buffered solution) buffer to remove the stain, the filters were blocked with 5% nonfat milk in TBS for 1 hour at room temperature, and then incubated with diluted primary antibodies overnight at 4°C.

At 2 and 4 h post-incubation, the glass slide chambers were completely washed with HBSS buffer to remove the non-specific binding particles.

Cells were washed with buffer to remove the unbound reagent.

The column was then washed three times with 1 ×  TBS buffer to remove the unbound dye.

Cells were then washed three times with 10 mM phosphate buffer to remove the pellets of flocculated yeast.

The filter was washed extensively in PBST buffer to remove the secondary antibody, and the blot was visualized with ECL reagent.

After 72 h the cells were pelleted by centrifugation and washed with 8 ml of PBS buffer to remove platensimycin and the pellet resuspended in fresh LB media.

The flow cell was extensively washed with buffer to remove free SSB protein from the flow cell before beginning the experiment.

The membrane was washed extensively in PBST buffer to remove any excess secondary antibodies, and the blot was visualized with enhanced chemiluminescence reagent (GE Healthcare, Piscataway, NJ).

The reaction mixture was filtered through the nitrocellulose membrane and washed 3 times with the selection buffer to remove unbound TAs.

Following 4 h of incubation, the resin was washed 3 times with 10 ml of the equilibration buffer to remove unbound proteins.