Sentence examples for buffer to recover from inspiring English sources

The beads were washed with exchange buffer to recover tag-free SeCitS and the protein was concentrated to 5 mg/ml (50 kDa cut-off).

After 45 minutes, a sample of the reaction mixture (about 1 mL) was passed through PD-10 column (Sephadex G-25 M, GE Healthcare, Uppsala, Sweden) using 5 mL of the same buffer to recover the protein fraction (HSA-sNHS).

As HRP binding with Si@poly APBA-PA) in the acidic buffer (0.1 M acetate, pH 4.6) iSi@poly APBA-PAin possible theuse this buffer to recover the bound glycoprotein from the immobilized polymer brushes.

Briefly, these sections were incubated with 1% H2O2 to inactivate endogenous peroxidase and put into 10 mM sodium citrate buffer solution to recover antigen.

When an error is detected in a flit buffer, its duplicated buffer is used to recover the correct value of the flit.

The cells were then washed to remove the loading buffer and allowed to recover in a pre-warmed growth medium.

A Zeba desalting column (Thermo Fisher Scientific Inc., Rockford, IL) pre-equilibrated with PBS coupling buffer was used to recover activated MECA32 mAb.

Nonetheless, one is still able to infer, with very high stringency, the degree/density of methylation based on the buffer conditions required to recover enriched DNA [ 14, 19].

For motility assays with AM141 [ rmIs133(P unc-54) Q40::YFP unc-54 5 adayt animals were individually placed in a drop of M9 buffer and allowed to recover for 30 seconds.

Cultures were then washed rapidly three times in Ca2+-free Tyrode buffer and subsequently allowed to recover under conditions that prevent synaptic activity, either Ca2+ free Tyrode buffer also containing 0.5 mM EGTA or Tyrode buffer containing 1 μM TTX.

Determining the buffer content is critical to recover the postpaint sequence.