Sentence examples for buffer to obtain the from inspiring English sources

Tetrahydrofuran was used as an additive in the running buffer to obtain the absolute ECL peak of bisoprolol.

After brief centrifugation, the cellular pellet was mixed with an equal volume of two-fold concentrated RIPA buffer to obtain the following final concentrations of detergents: 1% NP-40, 0.5% deoxycholate, 0.1% SDS.

Different quantities of penetratin were added to a 100 µl quartz cuvette containing 2 µg lipids in a HEPES 0.5 mM pH 7.4 buffer to obtain the desired peptide/lipid ratios and the absorbance was followed until it reached a plateau (30 minutes after peptide addition).

For each fractionation, the column was eluted with equilibration buffer to obtain the crude neutral polysaccharide fraction.

The pellets were washed twice with homogenization buffer and then sonicated in homogenization buffer to obtain the mitochondrial fractions.

Culture plate was washed with buffer A and was further extracted with warm 1 × sample buffer to obtain the Triton-insoluble fraction.

The precipitate was washed five times with Et2O/acetone (1 : 1) and was redispersed in distilled water or buffer to obtain an aqueous magnetic fluid [ 36].

The reaction solutions were prepared in 0.01 mol l−1 buffers to obtain the desired pH values.

One hundred milligrams of lung tissue was homogenized and fluidized in extraction buffer to obtain 5% of the homogenate.

Briefly, 0.5-mL semen samples were washed with phosphate-buffered saline (PBS), and the sperm were then resuspended in the binding buffer to obtain a cell density of approximately 5 × 10.

Then, agarose beads were collected by pulsing in a microcentrifuge and boiled in Laemmli buffer to obtain pull-down samples with the activated RAS.