Sentence examples for buffer to dilute from inspiring English sources

The emulsified mixture was collected into an airtight vial (containing 500 µL buffer to dilute and thus prevent protein synthesis from taking place in any cell-free mixture that avoids emulsification) and returned to the glove box immediately upon completion.

In the case of the 35% step, the encapsulated DNA nanostructure sample was used in place of 1× encapsulation buffer to dilute the 54% iodixanol solution.

The pooled assay mix was prepared by combining 8 of 20× TaqMan® Gene Expression Assays into a single tube and using 1× TE buffer to dilute the pooled assays to a final concentration of 0.2×.

Briefly, for ablation experiments, animals at specified days (5 dpf, except for Fig. 1B,C where treatment was performed at 2 dpf) were treated overnight (16 hours) with MTZ dissolved in DMSO after which they were rinsed extensively in E3 buffer to dilute MTZ over 1000-fold compared with the starting concentration.

In particular, it was noticed that the buffer used to dilute the bacteria was of importance.

For all analyses, the denatured proteins were diluted with buffer to reduce the salt concentration and digested with trypsin for 3 h at 37°C.

One μg of each labeled cRNA was fragmented by adding 5 μl 10 × blocking agent and 1 μl of 25 × fragmentation buffer, then the mixture was heated at 60°C for 30 minutes; finally, 25 μl 2 × GE hybridization buffer was added to dilute the labeled cRNA.

Blocking buffer was used to dilute primary and secondary antibodies.

Finally, 55 μl of 2× GE Hybridization buffer was added to dilute the fragmented RNA.

Finally, 55 μl 2× GE Hybridization buffer was added to dilute the labeled cRNA.

Membranes were blocked using 5% (w/v) non-fat milk/TBS-Tween 20, which was the buffer also used to dilute secondary antibodies.