Sentence examples for buffer to collect the from inspiring English sources

The samples were centrifuged at 380 g, and washed 5 times with IP buffer to collect the beads.

GFP-MAL2/HOG cells monolayers were washed in cold PBS, incubated 20 min in 1 ml of cold lysis buffer (50 mM Tris HCl pH 6.8, 150 mM NaCl, 1% Triton X-100) supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) and Complete Mini Protease Inhibitor Cocktail, and scraped in lysis buffer to collect the lysate.

Insoluble materials were dissolved in sodium dodecyl sulphate sample buffer to collect the nuclear extracts.

A needle tip was carefully submerged in a tube containing 50  µL of each extraction buffer to collect the cells.

Tumorsphere cells or adherent cells were lysed directly in nuclear lysis buffer to collect whole cell extracts.

The pellet was resuspended in 5 mL of buffer A, homogenized again in a Dounce homogenizer, and transferred to 1.5 mL of buffer AS to collect the nuclei by centrifugation.

After the loading of the sample in a total volume of 2.5 ml into the column, 3.5 ml of the buffer followed by another 2.5 ml of the buffer was added to collect the flow-through.

The caps containing buffer and cells were incubated upside-down at room temperature (RT) for 30 min and then briefly centrifuged to collect the buffer and cell complexes.

The bead protein complexes were washed three times with 100 μl of 1× RIPA buffer (10 mM Tris pH 7.5/1 mM EDTA/1%Triton X-100/0.1X-100/0.1%SDS/0.1%eoxycholate/100 mM NaCl) and once with 100 μl of TE (10 mM Tris pH 7.5/10 mM EDTA) buffer using a magnetic rack to collect the beads.

For preparation of nuclear lysates, hypotonic buffer followed by centrifugation was used to collect the cytosolic fraction (supernatant).

Thus, a simple filtration/extraction/centrifugation method was developed to collect the bacterial cells, extract the intracellular NADH using heat treatment in Tris buffer and collect the purified intracellular NADH fraction.