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Dried samples were then analyzed for moisture content in order to quantify the suitable amount of buffer to be added for enzymatic hydrolysis process [ 19].
Similar(59)
Ice-cold lysis buffer (modified RIPA buffer) was added to the cells (1 mL per 10 cells/100 mm dish/150 cm flask).
To each buffer was added 0.2% hydroxyethylcellulose to decrease electro-osmotic flow.
Then, samples were washed three times with RIPA buffer and SDS-loading sample buffer was added to the samples.
Subsequently, binding buffer (1×) was added to each tube.
During each period, a different buffer treatment was added to the diet.
Finally, 4× LDS reducing sample buffer (Invitrogen) was added to give 1× sample buffer concentrations.
Laemmli buffer (57) was added to the supernatants.
50 uL SureSelect neutralisation buffer was added to the buffer.
The substrate crude enzyme and buffer were added to tubes similar to the amylase assay.
Fragmentation buffer was added to interrupt mRNA to short fragments.
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