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The boric acid was added to electrolyte as a buffer to avoid the local pH rise caused by parallel hydrogen evolution reaction (HER).
HS stock solutions (1 g/L) were prepared freshly for each experiment following ref [31] but using 22 mM bicarbonate buffer instead of 50 mM phosphate buffer to avoid the potential formation of vivianite (Fe3(PO4 2·8H2O).
Cells were lysed with 8 M urea including 20 mM MMTS in the lysis buffer to avoid the trans-nitrosation followed by centrifugation at 12000 g.
The pellet (P20) was washed twice with RIPA buffer to avoid the presence of cytosolic proteins in the P20 sample and then resuspended in the original volume of the initial sample.
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Lysates can be prepared in sodium dodecyl sulfate (SDS -containing bufferSDS -containing proteases, buffersd to be dinactivatemilder buffers to avoid the denaturation of the anti-tag antibodies.
In all concentration steps the filters were washed with 0.1 M NaOH followed by careful rinsing with the appropriate buffer to avoid contamination of glycerol in the samples.
Whenever tests were performed for the action of PJ34, this substance was added to the cell lysis buffer to avoid inhibitor dilution as per the manufacturer's instructions.
We monitored the distribution of both proteins and lipids by SDS-PAGE using Bis-Tris gels run in MES buffer (to avoid excess counterions at the gel front that interfere with lipid staining).
If a received packet into the transceiver buffer cannot be attended by the microprocessor, it should be removed from the buffer to avoid overflow if possible.
We monitored the distribution of both proteins and lipids by SDS PAGE using Bis-Tris gels run in MES buffer (to avoid excess counter ions at the gel front which interfere with lipid staining).
We use regular growth media with serum (specific to the cells being used) as the flow buffer to avoid inducing flux, which can occur when using typical PBS-BSA flow buffers, but it should not matter if the cells are kept on ice.
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CEO of Professional Science Editing for Scientists @ prosciediting.com