Sentence examples for buffer to achieve the from inspiring English sources

A charge integrating amplifier with a gain adaptive to the number of incoming photons is combined with a correlated double sampling (CDS) buffer to achieve the required dynamic range and single photon sensitivity.

The solution was aliquoted and stored at –20 C. At the time of experiments, the stock solution of palmitate-BSA was diluted 1∶10 in the KRB or RPMI 1640 buffer to achieve the desired concentration of palmitate.

This solution was then diluted in HEPES buffer to achieve the desired aptamer concentration (1 μM, the optimal concentration for TGF-β1 binding).

Also, while activation is favored by organic solvents and acidic pH, 28 coupling requires aqueous buffer to achieve the template effect and neutral or basic pH to ensure nucleophilic reactivity at the primer terminus.

In view of the above problems, this paper presents the concept of particle buffer (PBuffer) to achieve the particle system management.

Lyophilized α-syn was dissolved in 20 mM Tris-HCl/150 mM NaCl/pH 7.4 (i.e.: the fibrillization buffer) to achieve a stock concentration of 3 mg/mL.

Owing to solubility limits of CB1954 in an aqueous solution, 5% DMSO was included in the reaction buffer to achieve CB1954 concentrations >1 m M. In separate experiments using 100  μ M CB1954, 5% DMSO was found to slow the rate at which NfsA and NfsB reduced CB1954 by 10 and 35%, respectively.

Because collagenase is a Ca2+-dependent enzyme, Ca2+ has to be added anew to the collagenase perfusion buffer to achieve effective enzymatic activity.

Thus we pursued optimization of the component and protocol of protein extraction buffer to achieve better extraction efficiency of proteins from formaldehyde-fixed and paraffin-embedded tissues.

Briefly, 50 μL of reaction mixture [0.1 M potassium phosphate, 1 mM EDTA, 0.5 mM DTT, 14.5 μL [I]rT3 (~ 4.9 μCi, 0.05 nmol)] was preincubated at 37°C for approximately 1 min. Fifty micro-liters of microsomes, diluted with liver homogenization buffer to achieve a final protein concentration of 0.5 mg/mL, was added to the reaction mixture.

For pH stability, the concentrated supernatants were mixed to the same volume of citric acid-sodium citrate buffer to achieve different pH values lower than 6.0 and with Tris-HCl buffer for pH higher than 7.0.