Sentence examples for buffer to a volume from inspiring English sources

Cells were then diluted with Annexin-binding buffer to a volume of 400 µL with propidium iodide.

After 5 washes with distilled water, triplicate wells were incubated at 37°C for 1 hr with various human sera samples diluted (1 1600) in blocking buffer to a volume of 100 μl.

Cells were then diluted with Annexin V-binding buffer to a volume of 150 μl and incubated with 1 μl FITC-labeled Annexin V (Bender MedSystems, Vienna, Austria) for 15 min in the dark.

The two cDNA targets were pooled together, and mixed with the hybridization buffer to a volume of 50 μl Dig Ease Hybridization buffer (Roche Applied Science, Mississauga, ON, Canada), 2.5 μl tRNA (Roche Applied Science) and 2.5 μl of Sonicated Salmon Sperm DNA (10 mg/ml; Invitrogen).

In a clean 1 ml quartz cuvette, 100 μl of a freshly prepared 120 μM htt solution in 50 mM Tris-HCl, 150 mM NaCl, and 1 mM DTT (pH 7.0) (htt-working buffer) was mixed with 100 μl of a 120 μM SERF1a solution that had been previously dialyzed against htt-working buffer, or with 100 μl htt-working buffer, to a volume of 200 μl.

The pellets containing sarkosyl-insoluble tau fractions were dissolved in SDS-PAGE loading buffer to a final volume corresponding to the 1/50 volume of the 1S supernatant.

To prepare low melting agarose plugs of HMW DNA, the pellet (~5×10) – be it of nuclei, protoplasts, germinated spores, or spores – was resuspended with the HMW DNA preparation buffer to a total volume of 0.6 mL, and an equal volume of 1% low melting agarose was then added to the buffer to a total volume of ca 1.2 mL at 45°C.

The pellets containing sarkosyl-insoluble tau fractions were dissolved in SDS-PAGE loading buffer to a final volume corresponding to the 1/50 of the volume of 1S supernatant used for their preparation.

The wells were filled with EGTA Mg GVB buffer to a final volume of 100 and 50 µL of 2 % RBC suspension was added.

Then the reactions were diluted with TE buffer to a final volume of 20 µl.

The sonicate was diluted 10-fold with IP Buffer to a final volume of 1 ml per immunoprecipitation reaction.