Sentence examples for buffer to a total from inspiring English sources

Three panels of assembly reactions were set up with 0.2 mM of each dNTP, 1 mM magnesium sulfate, 5 U of Pfu Turbo DNA polymerase, and differing amounts of oligonucleotide mix (1 µl, 2 µl, and 3 µl, respectively) in 1 X Pfu buffer to a total volume of 50 µl.

Typically, we would add 300 μL of buffer to a total of 4.5 μmol of lipid.

The preamplified solution was then diluted with 75 μl 0.1x TE buffer to a total volume of 100 μl.

Pellets were resuspended in TBS buffer to a total protein concentration of approximately 5 mg mL−1 and stored in liquid nitrogen.

Pellets were resuspended in PBS buffer to a total protein concentration of approximately 5 mg ml− 1 and stored in liquid nitrogen.

To prepare low melting agarose plugs of HMW DNA, the pellet (~5×10) – be it of nuclei, protoplasts, germinated spores, or spores – was resuspended with the HMW DNA preparation buffer to a total volume of 0.6 mL, and an equal volume of 1% low melting agarose was then added to the buffer to a total volume of ca 1.2 mL at 45°C.

50 µg protein was added to modified rehydration buffer (6M urea, 2M thiourea, 2% CHAPS, 2% IPG buffer) to produce a total volume of 125 µl.

Urine samples were diluted in assay buffer to obtain a total volume of 60 μl per well, and run in duplicate as previously described [ 13].

These samples were then incubated for 15 min at 37 °C, then diluted by the addition of 560 μL of 5 mM Tris-HCl, 1 mM EDTA, and 1 mM NaN3 (pH 8.0) buffer to give a total volume of 600 μL.

NAF was diluted with Tris buffer to a concentration of 3.6 mg total protein ml−1 Tris buffer.

For construction of Gateway®Entry vectors, 150 ng of pDONR207 was mixed with 2.5 μl of purified PCR product, 1 μl of Gateway® BP Clonase™ with TE buffer added to a total volume of 10 μl.