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Peptides were extensively lyophilized and dissolved in NMR buffer to a stock concentration of 110 mM.
Annealed DNA oligos (Eurogentec, Liege, Belgium) were lyophilized and dissolved in NMR buffer to a stock concentration of 11.5 mM.
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Ten minutes later, cells were placed into the Flexstation II (Molecular Devices, Sunnyvale, CA) where they received 20 μL of vehicle or test compound diluted into assay buffer to a 4× stock after 19 s, followed by 20 μL of glutamate diluted to a 5× stock at 109 s.
Compounds were diluted into assay buffer to a 2× stock using a Thermo Fisher Combi (Thermo Fisher, Waltham, MA), which was applied to cells at t = 3 s.
Lyophilized α-syn was dissolved in 20 mM Tris-HCl/150 mM NaCl/pH 7.4 (i.e.: the fibrillization buffer) to achieve a stock concentration of 3 mg/mL.
IFNβ, IL-1, and IL-6 standards were freshly prepared by reconstituting in the standard diluent buffer to give a stock concentration of 400 pg/ml.
For IFN-γ, standards were freshly prepared by reconstituting in the standard diluents buffer to give a stock concentration of 400 pg/ml.
Strong performance in nonbrokerage areas has been a buffer to stocks of other brokerage firms.
The ssDNA fragments were dissolved in nucleic acid binding NMR buffer to stock concentrations of 1 mM.
PaKynB and BcKynB were concentrated to 7.5 mg·ml−1 in buffer A1 and BaKynB to 4 mg· ml−1 in buffer A2 to provide a stock solution for crystallization experiments.
Before the enzymatic assay, Sac1 proteins from −80 °C storage were first diluted with reaction buffer to make an enzyme reaction stock (0.1 mg/mL or 1.8 μM).
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