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About 200 mg of the dry sample was dissolved in an acid mixture (equal parts of 11.6 M perchloric acid and 14.3 M nitric acid) and neutralized with citrate buffer to a pH 5.5 with an alkaline mixture of 7.8 M sodium hydroxide and 1.0 M trisodium citrate.
The eluted samples were immediately neutralized with Tris buffer to a pH of 7.5.
When setting the start buffer to a pH of 5.5 or 6.0 the maximum breakthrough is 6% and 3% respectively.
The supernatant containing DNA was removed and adjusted with HEPES buffer to a pH of 7 8.
The eluted samples were immediately neutralized with Tris buffer to a pH of 7.5 and the protein concentration was measured using the BCA protein kit.
Similar(55)
The filtrate was then centrifuged and buffered to a pH of 7.0 for analysis.
It becomes soluble when the injectable solution is buffered to a pH of 2.9 3.7.
Stock solutions were prepared in RPMI 1640 (Sigma) plates buffered to a pH of 7.0 with 0.165 M 3- N-morpholino) propanesulfonic acid (Sigma).
It was buffered to a pH of 7.6 by 50 mM of trihydroxymethylaminomethane (Tris -hydrochloric acid (Sigma-Aldrich, STris -hydrochloric.
Stock solutions were prepared in water and further diluted in RPMI 1640 medium (Sigma) buffered to a pH of 7 with 0.165 M 3- N-morpholino) propanesulfonic acid buffer (Sigma).
Stock solutions were prepared in dimethyl sulfoxide (voriconazole and amphotericin B) or water (fluconazole), and were further diluted in RPMI 1640 medium (Sigma) buffered to a pH level of 7 with 0.165 M 3- N-morpholino) propanesulfonic acid buffer (Sigma).
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