Sentence examples for buffer to a dilution from inspiring English sources

In brief, specific antibodies were diluted in blocking buffer to a dilution of 1 100 1 400.

The mixture was incubated at room temperature for 30 min, microcentrifuged at 10,000 g for 10 min, and 0.05 ml supernatant was neutralized with 0.025 ml 1 M Tris base and diluted with IGF-I assay buffer to a final dilution of 40 100 fold.

Briefly, after cells were treated with RAC1 inhibitor (100 μM NSC23766) for 24 and 48 h, all cells were collected and diluted with phosphate-buffered saline (PBS) containing 1% BSA as a dilution buffer to a concentration of 5 × 10 cells/mL.

After another three washes, MIF was detected by adding a biotinylated goat anti-human MIF antibody (R&D Systems) diluted in dilution buffer to a final concentration of 0.2 μg/ml and incubating for 2 hours at room temperature.

The sheared chromatin was diluted in ChIP dilution buffer to a final SDS concentration of 0.1%.

Sera were diluted in dilution buffer to an IgE antibody concentration in the range of 0.5 5 kUA/l, suitable for the indirect ELISA measurement.

The young leaf tissue from the field tree or from the greenhouse propagations is macerated with a chilled pestle and mortar in sufficient 0.05 M neutral potassium phosphate buffer to create a 1 10 dilution.

Fecal extractions diluted in blocking buffer to three dilutions (1 : 2, 1 : 20, and 1 : 200) were added (100  μL/well).

Serum samples were selected that showed comparable results in CENP-A and CENP-B ELISA and diluted in Dilution Buffer to obtain a reactivity of approximately 1.0 OD under standard assay conditions.

An amount of 0.025 mL of haemolysate was added with 12.5 mL of phosphate buffer to make a solution with 500x dilution.

Immediately prior to enzyme assays, liver, brain, and muscle supernatants were diluted in homogenization buffer to get an overall 100-fold dilution in relation to the starting tissue mass.