Sentence examples for buffer to a density from inspiring English sources
Cells were suspended in adhesion buffer to a density of 200,000 cells/ml.
The platelet pellet was resuspended in modified Tyrode's-Hepes buffer to a density of 2 × 10/ml.
Recover the bacteria by centrifugation at 4000× g for 10 min at room temperature and resuspend the cells with Infiltration Buffer to a density at OD600 = 1.0 ~ 1.5.
Next, cells were washed in 1 ml of annexin-binding buffer (10 mM HEPES, 140 mM NaCl, and 2.5 mM CaCl2, pH 7.4) and resuspended in annexin-binding buffer to a density of 1 × 10 cells/ml.
Cells were harvested in the mid-log phase of growth and washed twice in ice-cold Hepes-buffered saline solution (pH 7.4) supplemented with 0.1% (w/v) BSA and finally resuspended in this buffer to a density of 1 × 10 cells/mL.
Cells were cultivated in LBtet at 37 °C, 200 rpm for 16 h, harvested by centrifugation at 11,337×g for 10 min, washed twice and re-suspended in CPB buffer to an optical density (600 nm) of 10.
The pellet was washed by 0.05 M sodium carbonate buffer and then resuspended in the same buffer to an optical density of 1.0 at 640 nm.
Cells were counted and preadapted in PBS buffer at a density of 2.5 x 10 cells/ml (corresponding to two cells per leukocyte) incubating 30 min at 37°C before inoculation in human blood [ 22].
Cells were counted and resuspended in 1X annexin-binding buffer at a density of ∼1 × 10 cells per mL, preparing a sufficient volume to have 100 μL per assay.
Cells were washed twice in ice-cold PBS buffer and centrifuged at 900 rpm for 3 min. The pellets were resuspended in binding buffer at a density of 106 cells/mL.
