Sentence examples for buffer to a concentration from inspiring English sources

Briefly, after cells were treated with RAC1 inhibitor (100 μM NSC23766) for 24 and 48 h, all cells were collected and diluted with phosphate-buffered saline (PBS) containing 1% BSA as a dilution buffer to a concentration of 5 × 10 cells/mL.

They were washed three times with sodium acetate buffer in buffered solutions (sodium acetate, pH 4.75), and resuspended in 10 mL buffer to a concentration of ∼106 cells per mL.

Carefully resuspend the cells in cell binding buffer to a concentration of 2 × 106 cells/mL.

The washed cells were diluted in the same buffer to a concentration of OD600 = 1.0 (approximately 3.5 × 108 colony forming units (CFU) mL−1).

A lyophilized angiotensin-converting enzyme derived from porcine kidneys was reconstituted with an assay buffer to a concentration of 12.5 mU ml-1.

The oligos were resuspended in 1X buffer to a concentration of 20 µM.

Amplified libraries were diluted with Elution Buffer to a final concentration of 10 nM and run at a concentration of 4 6 pM on two Genome Analyzer (GAII) lanes.

Protein concentrations were measured using the Bio-Rad Protein Assay (Bio-Rad; Hercules, CA) and the concentrations in individual samples were equalized before adding 3x Laemmli buffer to a final concentration of 1x.

One more addition of buffer and concentration was performed, after which the protein was diluted with additional sodium phosphate buffer to a final concentration of 0.3 mg /ml.

After the protein concentrations were determined, the supernatants were mixed with 4X sample buffer and lysis buffer to a final concentration of 1 mg/mL protein.

Tissues were homogenized using a polytron (Kinematica AG, Lucerne, Switzerland) in a cold Tris HCl 50-mM (pH 7.0) buffer to a final concentration of 50 mg/mL.