Sentence examples for buffer the tissue was from inspiring English sources

After rinsing in same buffer the tissue was dehydrated through a series of graded ethanol to propylene oxide, infiltrated and embedded in epoxy resin and polymerized at 70°C overnight.

After rinsing in phosphate buffer the tissue was trimmed into 2 mm thick transverse slices.

After rinsing in cacodylate buffer, the tissue was postfixed in 1% cacodylate buffered osmium tetroxide for 2 h at room temperature, then dehydrated in a graded series of ethanol, quickly transferred to propylene oxide and embedded in Epon-Araldite.

After rinsing with phosphate buffered saline, the tissue was treated with 4'-6-diamidino-2-phenylindole (DAPI, Invitrogen) for nuclear staining.

After another brief rinse with Tris-buffer, the tissue was incubated with the biotinylated secondary antibody for another 25 min.

Slides were removed from labelling buffer and excess buffer surrounding the tissue was wiped away.

After rinsing with Buffer A, the tissue was chopped repeatedly with razor blades until a homogenous texture was achieved.

Samples were suspended in 0.05% RapiGest (Waters, Milford, MA, USA) buffer, and the tissue was homogenized and sonicated for protein extraction.

The breast cancer specimens used in this study were fixed with 4% buffered formalin, and the tissue was then embedded in paraffin.

After rinsing in 0.1 M NaPO4 buffer at pH 7.4, the tissue was post-fixed in 2% osmium tetroxide in 0.1 M NaPO4 buffer at pH 7.4 for one hour.

RNAlater was replaced in the 2 ml plastic tube with 1.0 ml of RLT buffer (Qiagen GmbH., Germany), and the tissue was homogenized by adding a 5 mm diameter Zirconium bead (Funakoshi, Japan) and shaking with a MixerMill 300 (Qiagen GmbH., Germany) at a speed of 20 Hz for 5 min (only the outermost row of the shaker box was used).