Sentence examples for buffer the supernatant was from inspiring English sources

After incubation in this buffer, the supernatant was collected (S) and the insoluble proteins were pelleted.

Cells were lysed with RIPA sample buffer, the supernatant was collected and protein concentration was determined using a Pierce protein assay kit (Thermo Scientific).

The protein G-Sepharose beads were then washed three times with a lysis buffer, boiled in 30  μl of SDS-sample buffer, and the supernatant was analyzed by immunoblotting with monoclonal anti-RUNX2 antibody.

The intact nuclei were collected by centrifugation at 200×g and washed twice in buffer A. The supernatant was centrifuged at 10,000×g for 10 minutes, and the resulting supernatant was fractioned into cytosol and plasma membrane fractions by centrifugation at 200,000 g for 1 hour.

Samples were washed in binding buffer, and the supernatant was discarded.

The resulting pellet was dissolved in 1.2× non-reducing Laemmli sample buffer and the supernatant was concentrated as described above.

The 20% (w/v) liver homogenates was prepared in the 0.1 M Tris-HCl buffer and the supernatant was used for biochemical analysis.

In brief, the cells were lyzed immediately at the end of exposures (kit-included lysis buffer) and the supernatant was stored at −80°C until analysis.

Genomic DNA was extracted by heat inactivation of culture material dissolved in TE buffer and the supernatant was used directly as template.

The resulting supernatant was saved and the phage pellet resuspended in 0.6 ml of Buffer A. The supernatant was centrifuged a second time to collect any remaining phages for 4 hrs at 73,000 × g.

Briefly cells were harvested and treated with 100 μl lysis buffer and the supernatant was incubated for 2 h with RNase A at 56°C and followed by Proteinase K digestion at 37°C for 2 h.