Sentence examples for buffer the specimens were from inspiring English sources
After being washed in 0.1 M cacodylate buffer, the specimens were post-fixed in 1% osmium tetroxide in 0.1 M potassium ferricyanide for 1 h and dehydrated in an ascending series of ethanol concentrations ranging from 30% to 100%.
After further washes with the above buffer, the specimens were collected and embedded in agarose.
After three 5-minute washes in the same buffer, the specimens were incubated in the secondary antibodies under the same conditions.
After washing in the same buffer, the specimens were dehydrated in a graded acetone series, critical-point dried with CO2, sputter-coated with a thin layer of gold and observed under a Zeiss DSM 962 SEM operating at 15 kV.
After post-fixation for one day in 4%% paraformaldehyde in 0.1 M phosphate buffer (PB), the specimens were cryoprotected using 10%, 200 %and30%0 % glucose in 0.1 M PB.
Following three rinses in 0.15 M sodium phosphate buffer (pH 7.2) the specimens were post-fixed in 1% OsO4 in 0.12 M sodium cacodylic buffer for 2 hours.
After washing with 100 mM phosphate buffer (pH 7.2), the specimens were post-fixed with 1 % osmium tetroxide overnight at 4 °C.
After 2 washes with 0.2 M sucrose in 0.1 M Na-cacodylate buffer, pH 7.2, the specimens were then postfixed with 1% osmium tetroxide containing 1.5% potassium cyanoferrate, dehydrated in gradual ethanol (30 100%) and embedded in Epon.
Following deparaffinization and antigen retrieval in citrate buffer (pH 6.0), the specimens were incubated with primary antibodies followed by horseradish peroxidase (HRP -conjugated secondary antibodies and tHRP -conjugatedwith the 3-amino-9-ethylcarbazole (AEC) + kit (Agilent Technologiesecondaryokyo, Jantibodies
The specimens were then transferred to buffer and processed routinely for embedding in Paraplast® (Merck, Darmstadt, Germany).
After washing with Tris-buffered saline containing 0.1%Tween-20Tween-20), the specimens were sequentially incubated for 10 30 min with biotinylated anti-rabbit and anti-mouse immunoglobulins and peroxidase-labelled streptavidine.
