Sentence examples for buffer the slide was from inspiring English sources
After three 1 h washes with PBST buffer, the slide was dried under N2 and a silicon mat was attached.
After washing with GE washing buffer, the slide was scanned with Agilent Microarray Scanner G2565BA (Agilent Technologies) at 5- μm resolution.
Biofilms on glass slides were also quantified using Crystal Violet staining; after washing in sterile phosphate buffer the slides were coated with 1 mL of Crystal Violet solution (0.1% (w/v) for 15 min).
Six of the twelve slide/gasket assemblies were sequentially disassembled into the first staining dish containing Wash Buffer 1. Once disassembled, the slide was quickly transferred into a slide rack in a second staining dish containing Wash Buffer 1.
The slide was washed twice with PBST buffer for 30 min. The slide was stored in PBS (pH 7.4) at 4 °C before use.
After boiling in Tris pH 8 containing buffer H2 for 30 min, the slide was incubated for 30 min at RT with the mouse monoclonal anti-PTEN ab clone 6H2.1 (1 200 dilution, DAKO-Cytomation, Glostrup, DK).
Following a series of washes in borate buffer, the slides were mounted in borate buffer for immediate imaging.
After rinses in 0.1% TBST and rinses in borate buffer, the slides were re-blocked in 2% BSA in borate buffer at RT for 15 minutes.
After rinsing in wash buffer, the slides were incubated with HRP conjugated appropriate secondary antibody.
After rinsing in wash buffer, the slides were then treated with labelled polymer, HRP, according to the manufacturer's instructions.
