Sentence examples for buffer the sections were from inspiring English sources

Following osmication with 1% osmiumtetroxide in cacodylate buffer the sections were dehydrated using ascending alcohol concentration steps, followed by two rinses in propylene oxide.

After further washing on 10 drops of incubation buffer, the sections were postfixed in 2% glutaraldehyde.

After being washed with the blocking buffer, the sections were incubated with the appropriate secondary antibody for 1 h at room temperature and extensively washed with PBS.

Immunolabelling: After a blocking step of two times 10 mn in glycine (0,5 mM in PBS) and another 10 mn in 1% BSA/PBS (blocking buffer), the sections were incubated 20 min with the primary antibodiy.

After washing 3 times in 0.05M Tris buffer, the sections were incubated with secondary antibodies (see Table 2) for 1 hr at RT. Nucleic acid staining was carried out by labeling with Bisbenzamide for 10 minutes.

After antigen retrieval in citrate buffer, the sections were blocked overnight at 4°C.

After washing with a Tris buffer solution, the sections were incubated with EGFR for 1 hour at room temperature.

After washing in Phosphate Buffer Saline, the sections were incubated with Cy���2-conjugated AffiniPure donkey anti-mouse IgG (Jackson Immunoresearch) for 1 h at room temperature.

After washing, the endogenous peroxidase activity was blocked by incubation at room temperature in 3% H2O2 for 5 min. After washing with the buffer solution, the sections were incubated with a labelled polymer-HRP goat anti-rabbit (CD3 and CD20) or goat anti-mouse (MAC387) for 30 min at room temperature.

For immunohistochemistry, antigens were retrieved in citrate buffer, and the sections were blocked with 3%% hydrogen peroxide and incubated with a primary antibody against p57 (1 100, sc-56341, Santa Cruz Biotechnology, Santa Cruz, CA, USA) and anti-p-cofilin (Ser3) antibody (1 100, 11139, Signalway Antibody, Pearland, TX, USA) overnight at 4 °C.

Briefly, after deparaffinizing and hydrating with phosphate-buffered saline (PBS) buffer (pH 7.4), the sections were pretreated with hydrogen peroxide (3%) for 10 minutes to remove endogenous peroxidase, followed by antigen retrieval via steam bath for 20 minutes in EDTA.