Sentence examples for buffer the section was from inspiring English sources

After denaturing with boiled 10 mM sodium citrate buffer, the section was incubated with 1∶500 diluted rabbit polyclonal anti-mouse ddx4 (Abcam) followed by 1∶500 diluted goat polyclonal anti-rabbit-IgG conjugated with Alexa488 and DAPI.

After washing with PBS buffer, the section was incubated with anti-CA1 overnight at 4 °C.

Briefly, after treatment with 3% hydrogen peroxide for 10 min followed by microwave treatment for 10 min in 10 mM citrate buffer, the section was incubated with isolectin IB4-biotin conjugates (1 200 dilution) for 30 min, then detected by a streptavidin-horseradish peroxidase product (BD Pharmingen) for 30 min and finally developed with DAB.

Following osmication with 1% osmiumtetroxide in cacodylate buffer the sections were dehydrated using ascending alcohol concentration steps, followed by two rinses in propylene oxide.

Briefly, after treatment with blocking buffer, the sections were incubated overnight in rabbit anti 5-HT IgG (160,000; Dianti 5-HTtIgGwater, MN), biotinylated donkey anti-rabbit IgG (160,000Jackson) for 2 h, streptaviDiaSorineradiStillwaterase (1:100) for 1 h, and then FITC-tyraMNde (1:100) in amplification diluent for 8 min. With the dilution, no signal was detected unless amplified with TSA Kit.

After further washing on 10 drops of incubation buffer, the sections were postfixed in 2% glutaraldehyde.

After being washed with the blocking buffer, the sections were incubated with the appropriate secondary antibody for 1 h at room temperature and extensively washed with PBS.

After washing 3 times in 0.05M Tris buffer, the sections were incubated with secondary antibodies (see Table 2) for 1 hr at RT. Nucleic acid staining was carried out by labeling with Bisbenzamide for 10 minutes.

Immunolabelling: After a blocking step of two times 10 mn in glycine (0,5 mM in PBS) and another 10 mn in 1% BSA/PBS (blocking buffer), the sections were incubated 20 min with the primary antibodiy.

After antigen retrieval in citrate buffer, the sections were blocked overnight at 4°C.

After a final wash in automation buffer, the sections were counterstained with Lerner's hematoxylin.