Sentence examples for buffer the samples were from inspiring English sources

After supplementation of 25 µl of protein sample loading buffer the samples were boiled for 5 min.

After the addition of SDS-PAGE buffer the samples were heated to 95°C and analyzed by Western blot using an anti-HA antiserum.

After washing with PIPES buffer, the samples were stained with 1% osmium tetroxide (OsO4) (Sigma Aldrich, USA), washed twice with PIPES buffer and dehydrated by immersing in an ethanol series.

After filtration and washing with the phosphate buffer, the samples were utilized to immobilize HRP.

After three washes with NP-40 lysis buffer, the samples were boiled with 2× SDS loading buffer and subjected to SDS-polyacrylamide gel electrophoresis (PAGE) and Western blotting analysis.

Following washing in NP-40 lysis buffer, the samples were processed for Western blotting.

After washing in 4% buffered formalin, the samples were dehydrated and embedded in paraffin.

The immunoprecipitate was then washed three times with lysis buffer, resuspended in 20 μl of SDS sample buffer, and the samples were heated for 5 min at 100°C.

After washing three times with the same lysis buffer, the beads were re-suspended in the sample buffer and the samples were separated by SDS-PAGE.

After overnight dialysis against 100 mM sodium phosphate buffer, pH7.0, the samples were concentrated using Amicon Ultra-15 100,000 MWCO spin concentrators as described by the manufacturer.

Ammonium sulphate was added as a buffer, and the samples were neutralised with NaOH (10 M), and then the mixture was extracted twice with ethyl acetate.