Sentence examples for buffer the sample was from inspiring English sources
After 3 washes with wash buffer the sample was fixed with 2% glutaraldehyde for 5 minutes before staining with 2% uranyl acetate.
After 30 min, the controller switched off automatically and the cathode chamber buffer (the sample) was emptied into an Eppendorf vial.
After washing with 0.1 M phosphate buffer, the sample was dehydrated by a series of incubations in 30, 50 and 70% (v/v) ethanol.
After washing with 0.1 M phosphate buffer, the sample was dehydrated by a series of incubations in 30%, 50% and 70%, ethanol.
After dialysis against the Tris-HCl buffer, the sample was further purified to remove endotoxin by polymyxin B column (Bio-Rad, USA).
After the addition of SDS-PAGE buffer the samples were heated to 95°C and analyzed by Western blot using an anti-HA antiserum.
After lysis with RIPA buffer, the sample were divided in two parts, one were precipitated with acidic phenol/chloroform (pH 4.5) for RNA extraction and the other were used for differential centrifugation followed by Western blot as described before.
Although, the yield of a new LRRK2 purification carried out using the adapted buffers, HEPES buffer at pH 6.8, for cell lysis and protein extraction by affinity binding, and HEPES buffer at pH 8.2 to elute it from the affinity column, was comparable to the yield of control purifications carried out under the initial conditions with Tris/HCl buffers, the sample was of higher quality.
After purification with the Ni-NTA column, the buffer of the sample was exchanged with buffer D or 100 mM ammonium acetate buffer (pH 6.6) by ultrafiltration for the acceptor screening or LC-MS analysis of the donor specificity, respectively.
The buffer of this sample was exchanged with buffer C. The sample was further purified by anion exchange and size exclusion chromatography as described for the kinetic analysis, except that the eluted sample of the size exclusion column was just concentrated for crystallization.
