Sentence examples for buffer the reaction was from inspiring English sources

By adding 500 μL of the particular substrate solution (10 mM in catalysis buffer), the reaction was started.

Immunocomplexes were washed After being washed for 3 times in RIPA buffer, the reaction was terminated by adding 5× SDS sample buffer and then subjected to western analysis.

Briefly, 50 μg of cell extract was incubated in reducing buffer (50 mM HEPES, 1 mM EDTA, 1 mg/ml BSA and 2 mM DTT) at 37°C for 15 min. After the addition of reaction buffer, the reaction was started by the addition of 5 μl bovine thioredoxin reductase (100 U/ml) or water as negative control followed by 20 min incubation.

Addition of a 3' terminus adenine to the fragments was done in 30 μl using 21 μl of the previous reaction, 0.1 mM dATP, 10 U of Klenow exo minus (Epicentre) and 3 μl of 1x T4 DNA Ligase buffer; the reaction was incubated for 30 min at 37°C and then 20 min at 75°C.

For N-α-tosyl l-lysinyl-chloromethylketone (TLC K inhibition, 5.5 μg of rabbit proteasome fraction II or 7.8 μg of control yeast deficient lysate was incubated with TLCK in buffer, pH 5.5, at 37 °C for 30 min. The incubation was diluted 1 10 in assay buffer, and the reaction was initiated with 0 5 μM proteasome sensors.

For the assay with the purified tubulin (>99% from Cytoskeleton, Inc), the tubulin solution was diluted to a concentration of 10 µg/ml in the assay buffer, and the reaction was performed essentially the same as the cell lysate.

Complex IV (cytochrome c oxidase) activity was determined by the change in absorbance at 550 nm (ε = 19.1 mM−1cm−1) due to the oxidation of reduced cytochrome c. 15 µM reduced cytochrome c and mitochondria were added to the assay buffer and the reaction was followed for 2 minutes.

dATP overhangs were added to the DNA using 15 µL of purified library template, dATP (1 µL 100 mM), 15 units Klenow exo- (NEB) and 2 µL NEB buffer 2. The reaction was incubated at 37°C for 30 minutes, then purified with a MinElute column and eluted in 21 µL of water.

Reaction mixture contained 100 µl of culture liquid, 870 µl MOPS (3- N-morpholino propanesulfonic acid) buffer and the reaction was started by the addition of 3- N-morpholino propanesulfonic

For the TRAP kinetic assay, the method was similar but the samples were incubated in a sodium acetate 50 mM and sodium tartrate 10 mM (pH 4.8) buffer and the reaction was stopped with NaOH 0.1N.

Subsequently, the parasites were removed by centrifugation (2000  g, 5 min) and the absorbance of the reaction product [ p-nitrophenolate (pNP)] was measured in the supernatant at 405 nm; (ii) HeLa cells expressing the r LdMAcP His chimera or the empty vector were incubated with substrate buffer and the reaction was terminated.