Sentence examples for buffer the protocol was from inspiring English sources

After washing with PBS/BSA buffer, the protocol was followed according to the manufacturer's instructions with two modifications: the reactions occurred at room temperature, and the incubation times were duplicated, except after the addition of the color reagent.

The hybridization buffer mentioned in the protocol was replaced by ULTRAhybTM Ultrasensitive Hybridization Buffer (Applied Biosystems/Ambion, Austin, TX).

The protocol was adapted from Nature protocols.

A practical approach to increase the mobility tolerance of the protocol is applying a buffer zone to the transmission ranges of the nodes.

The pH 6.0 citrate buffer antigen retrieval protocol was applied for the antibody.

"The protocols were quite clear.

By varying the composition of PEG in the precipitation buffer, the protocol can be adjusted to specifically suit the fragment length appropriate for the starting material, or adjusted to suit the read-length of the sequencing system.

The buffers and protocol are similar to those described elsewhere [ 38, 39].

The only minor deviation to the standard protocol was that elution buffer volume was adjusted to 25 μL or 100 μL depending on the amount of leaf material used.

Fifty microliters of DNA extraction buffer solution from each protocol was added to the deparaffinized tissue in a 1.5 mL microcentrifuge tube followed by the DNA extraction step.

For DNA from urine, a few drops of urine were dispensed into an Eppendorf tube containing 200 µl of ATL buffer and the manufacturer's protocol was applied.