Sentence examples for buffer the protein was from inspiring English sources

After the column had been washed with the same buffer, the protein was eluted by addition of the same buffer containing 0.05 mol l-1 NaCl.

Following a 24-hour dialysis against buffer, the protein was 77% octameric and had 74% of its original activity.

After dialysis against 10 mM imidazole, pH 7.4, 1 mM EGTA, 0.1 mM EDTA, and 1 mM DTT buffer, the protein was applied onto a DEAE cellulose column equilibrated in the same buffer.

After washing with 5 bed volumes of the loading buffer, the protein was eluted over a 0 500 mM NaCl gradient.

The cell lysate was cleared by centrifugation and the supernatant was applied onto a GST-trap column (GE Healthcare) pre-equilibrated in buffer A. The protein was eluted with buffer A containing 10 mM of reduced glutathione.

Before each experiment, aliquots of the LC were thawed to room temperature and immediately passed through a PD-10 gel-filtration column equilibrated with Buffer P. The protein was collected in Buffer P and stored on ice.

After 13 column-volume buffer wash, the protein was eluted with a buffer containing 25 mM HEPES (pH 7.8), 200 mM NaCl, 5% glycerol, 500 mM imidazole, 0.1 mM TCEP and 0.05% (w/v) DDM.

Non-specific contaminants were washed away with 25 mM imidazole in DM buffer and the protein was eluted in DM buffer containing 300 mM imidazole.

Finally the buffer of the protein was changed to 1 mM potassium phosphate buffer, pH 7, by two series of dialysis and concentrated to 850 µM.

The beads were washed 5 times with lysis buffer, and the protein was eluted by boiling for 5 minutes in 4X sample buffer.

For protein quantification the cells were lysed by a CHAPS-based lysis buffer and the protein was estimated by the DC Protein Assay kit from BIO-RAD according to manufacturer's protocol.