The remaining splenocyte suspension was resuspended with 2-ml Tris-NH4Cl buffer solution (the proportion of 0.16 mol/l NH4Cl and 0.17 mol/l Tris was 9 1, pH 7.2) to lyse red blood cells.
Conversely, the convex hull and rectangular boundaries enclose less than half of Al-Madinah without a buffer zone, and thus when the buffer is added, the proportion of the city within the boundary increases sufficiently to also increase the eruption probability, even though the spatial intensity at any specific point is reduced (Table 3).
The fermented material was manually homogenized and weighed (around 1 g) and the extraction buffer was added in the proportion of 1 : 10 (w/w).
While the anticoagulant strength of the citrate solution depends on the citrate concentration, the buffer strength depends on the proportion of strong cations in the fluid counterbalancing the citrate anion.
The distribution (median and IQR) of the districts' sociodemographic indicators were described for the districts within and outside 50 km buffer; these indicators included the proportion of rural population, ranking, proportion of population with low SOLI and proportion of people living below the poverty line.
LUM land-use mix entropy is measured by {text{LUM}} = (( - 1)/{text{ln}};n Sigma {text{Pi}}^*{text{ ln Pi,}}where n is the number of different land uses in 500 m buffers and Pi is the proportion of land in type i.
Figure 1b shows an example of buffers used to assess proximity of a homestead to the wetland, where proximity is expressed as the proportion of the buffer intersecting with the wetland area polygon.
These counts were divided by the proportion of the buffer overlapping the DNA survey grid, and by the probability of capture (derived from data of the closest observed distance of GPS collared bears to known bait sites – see 67), which varied by the age, sex and reproductive status of the individual being detected, and the DNA survey stratum [ 68].
Another recent study (focusing on Rhodesian HAT in Uganda) investigated the significance of the proportion of different sized buffer zones (circular zones, of defined radius, centred on a point of interest) surrounding homesteads that intersected with areas of wetland for HAT acquisition.
An extraction buffer (4 % SDS, 2%% β-mercaptoethanol, 2 mM PMSF, 100 mM TrisHCl, pH 8,8) was added in the proportion of 10 μl of extraction buffer per 1 mg of powder mass.
