Sentence examples for buffer the plates were from inspiring English sources

After washes with ice-cold binding buffer, the plates were dried and filled with Microscint-o (PerkinElmer).

During the incubation period with the lysis buffer, the plates were swirled approximately every 5 minutes to homogenize the constituents present in the wells.

After removal of the Blocking Buffer, the plates were washed three times with PBS.

After blocking with 2% BSA in carbonate buffer, the plates were washed 3 times with PBS-T (1 × PBS, 0.05% Tween 20, pH 7.2).

After washing three times with PBS, 0.5% (v/v) Tween-20 (washing buffer), the plates were incubated with 0.17 μg/ml biotinylated anti-porcine IFN-γ Ab (P2C11, BD Biosciences, Allschwil, Switzerland) in PBS at 4°C O/N.

After two washes with binding buffer, the plates were incubated with I-RGDyK (2 nmol/L) in the presence of various concentrations of ligand ranging from 1 pM to 10 μM for 16 h at room temperature, followed by three washes with binding buffer.

After washing four times with PBS containing 0.05% Tween-20 buffer (PBST), the plates were blocked with 300  μl/well blocking buffer (Hank's buffered salt solution, pH 7.0, 25 mM HEPES, 1% bovine serum albumin, 0.1% ovalbumin, 0.1% non-fat dry milk, 0.001% NaN3) for 1 h at room temperature.

cAMP was extracted by adding 40 μL of lysis buffer, and the plates were gently shaken at room temperature for 15 min.

For luciferase measurement, sample wells were washed twice with phosphate-buffered saline, followed by addition of cell lysis buffer (Promega, Madison, WI); the plates were then shaken for 20 min at room temperature to allow cell lysis.

The blocking buffer was removed, and the plates were washed and incubated with a solution of 1  μg/mL of fibronectin (Sigma) in IB buffer by 1 h at 37°C.

After washing, 100 µL of RatαHOSP-Ig diluted 1∶1000 in sample buffer was added and the plates were again incubated for 1 hr at 37°C on a rocker before being washed three additional times.