Sentence examples for buffer the plate was from inspiring English sources

After a total of three washes with wash buffer, the plate was blocked with reagent diluent and then incubated at room temperature for 2 hours for sample addition.

Following 2 washes with 1X wash buffer, the plate was blocked with 300  μL of 1X General Blocker Buffer for 3 6 h at room temperature.

Following addition of enzymes, sodium azide, and buffer, the plate was re-sealed and placed in an incubation shaker (Multitron Infors-HT, ATR Biotech, Laurel, MD) at 50°C, 150 rpm for 72 h.

After washing 5 times with 0.12 M NaCl, 0.04 M sodium phosphate, pH 7.2 buffer (PBS), the plate was blocked by adding 100 μl of blocking buffer (2% w/v gelatin in PBS) for 1 h at 37°C.

In a high protein binding 96-well plate (Falcon), 10 µg of protein was loaded in a total of 50 µL homogenization buffer and the plate was incubated at 4°C overnight.

One hundred microlitres of Hoechst stain 33258 was added to each well (1 mg of bis-benzimide 33258 in deionised water, further diluted to 1 : 50 in TNE buffer), and the plate was agitated.

The coating buffer was discarded and the plate was machine washed (SkanWasher; Skatron, Sterling, VA) with wash buffer (0.5% Tween 20 in phosphate-buffered saline) (Sigma-Aldrich).

After the final wash, all buffer was removed and the plate was removed from the magnet.

Dye was removed, 20 μL assay buffer was added, and the plate was incubated for 10 m at room temperature.

Biotinylated 211 diluted to 1 μg/ml in blocking buffer was added, and the plate was incubated at 37°C for 2 hours.

To perform the assay, 20 μL 10 U/mL of enzyme in appropriate buffer was added and the plate was incubated overnight at 30°C, after which halos were examined.