Sentence examples for buffer the pellets were from inspiring English sources

After an additional wash in ELB buffer, the pellets were suspended in ELB buffer and sonicated.

Then, they were centrifuged at 14 000 rpm for 30 min. After removal of the supernatant containing the lysis buffer, the pellets were transferred to the stainless steel MALDI-MS sample carrier.

After washing three times with ice-cold EBC buffer, the pellets were suspended in 2× loading buffer (125 mmol/l Tris.HCl, pH 6.8, 4% SDS, 20% glycerol, 14.4 mmol/l 2-mercaptoethanol) and boiled for 5 min. Immunoblotting was carried out with anti-human APC3, anti-human APC6 (Santa Cruz Biotechnology), or anti-mouse APC7 antibodies.

The mitochondrial pellets were centrifuged again with fresh homogenization buffer and the pellets were suspended in Tris-HCl pH 7.4, 150 mM NaCl to react with protease, and subjected to proteinase K assay.

To avoid contamination with soluble enzymes, the resulting pellets were washed three times by suspension in 10 mM Tris-HCl buffer at pH 7.4 The pellets were then homogenized in 10 mM Tris-HCl buffer at pH 7.4, and centrifuged at low speed (800 ×g) for 3 min to purify the samples.

Cells were collected and washed twice with phosphate buffer saline (PBS), and the pellets were incubated with 500 μM Rh123 in the dark for 10 min at room temperature.

After rinsing three times with the buffer, the pellet was then dehydrated through a graded ethanol series and n-butylglycidylether (Nisshin EM, Tokyo, Japan), and embedded in Spurr's resin.

The pellets were then buffered to label MEFs.

Pellets were resuspended, homogenized, centrifuged, and the pellets were resuspended in buffer.

The pellets were dissolved in RIPA buffer.

The pellets were resuspended in RIPA buffer.