Sentence examples for buffer the nuclei were from inspiring English sources

After six washes with washing buffer the nuclei were stained with 1 µg/ml DAPI for 30 sec in PBS.

After three washes in hypotonic buffer, the nuclei were resuspended in lysis buffer and subjected to three rounds of sonication on a Digital Sonifier (Branson) for 12″ at 20% power input with 2′ on ice between each cycle.

The reaction was stopped by using working strength stop/wash buffer, and the nuclei were counterstained with Hoechst 33342.

Cells were lysed with a hypotonic lysis buffer, and the nuclei were pelleted by centrifugation for 10 min, 4°C, at 2400 g.

Unbound secondary antibody was removed by washing three times with washing buffer for 5 min and the nuclei were counterstained with PI (2 μg/mL) for 2 min in the dark.

The nuclei were washed in buffer A, without NP40, supplemented with 20%% glycerol and 0.3 M sucrose and resuspended in 200 μl buffer C: 10 mM Tris HCl (pH 7.4), 15 mM NaCl, 3 mM MgCl2, 0.6 mM CaCl2, 0.5 mM DTT and 0.25 M sucrose.

After rinsing the structures with one wash of IF buffer and 2 3 washes of PBS, the nuclei were counterstained with To-Pro-3 (5 μM, Molecular Probes, Eugene, OR)or DAPI (4′,6-diamidino-2-phenylindole) (Sigma).

The nuclei were extracted using Buffer C for 40 minutes on ice.

The nuclei were resuspended in buffer A (20 m M HEPES-NaOH, pH 7.9; 400 m M KCl; 1 m M EDTA; 1 m M EGTA; 0.02% Tween 20; 10% glycerol; 1 m M dithiothreitol (DTT); 1 m M phenylmethylsulfonyl fluoride (PMSF); 1 μg ml−1 of leupeptin, pepstatin and antipain, respectively) and agitated with a stirring bar for 30 min followed by centrifugation at 100 000  g for 60 min.

The nuclei were resuspended in extraction buffer to a final concentration of 0.39 mol/L KCl and centrifuged at 100,000×g for 30 minutes.

The nuclei were washed with TM2 buffer to remove detergent once.