Sentence examples for buffer the mixtures were from inspiring English sources

When the most abundant free nucleosides and bases detected in the DR-ultrafiltrate (Table S1) were combined with Mn2+ and phosphate buffer, the mixtures were not as radioprotective of BamHI as the DR-ultrafiltrate (Figure 3A, gels 16 19).

STIV (10 TCID50/ml) was incubated with each aptamer (200 nM) in binding buffer and the mixtures were passed through a pre-wetted 0.1-μm PVDF filter (EMD Millipore).

The assays employed 10 μL of diluted centrifugation supernatant and 90 μL of the respective pNP (0.5 mM, diluted in citrate buffer), and the mixtures were incubated for 10 min at 50°C.

Then again 1 ml cold Hank's buffer was added and the mixtures were centrifuged for 5 min at 10,000 rpms.

After adding buffer C and mixing well, the mixtures were centrifuged at 700 g for 10 minutes at 4°C.

After the buffer was changed to separation buffer, the mixture was incubated at 37°C for 10 min.

After addition of magnesium sulphate, sodium chloride and citrate buffer, the mixture is shaken intensively and centrifuged for phase separation.

After washing away excess GST protein with 5 volumes of coupling buffer, the mixture was transferred to 0.1 M Tris-HCl buffer, pH 8.0 and incubated at room temperature for 2 h.

After adding 20  μl proteinase K and 200  μl AL buffer, the mixture was incubated at 56°C for 10 min.

The solution was mixed with equal volume of 66% sucrose in MBS buffer, the mixture was placed at the bottom of an ultracentrifuge tube, and a discontinuous gradient was formed by overlaying with the 30% sucrose and 5% sucrose solutions.

Subsequently, the starch suspension was layered on the top of a 5 ml cushion consisting of 90% (v/v) Percoll (GE Healthcare Bio-Sciences AB, Uppsala, Sweden) and 10% (v/v) extraction buffer, the mixture was centrifuged for 15 min at 400 g.