Sentence examples for buffer the mixture was from inspiring English sources

After the buffer was changed to separation buffer, the mixture was incubated at 37°C for 10 min.

After washing away excess GST protein with 5 volumes of coupling buffer, the mixture was transferred to 0.1 M Tris-HCl buffer, pH 8.0 and incubated at room temperature for 2 h.

After adding 20  μl proteinase K and 200  μl AL buffer, the mixture was incubated at 56°C for 10 min.

The solution was mixed with equal volume of 66% sucrose in MBS buffer, the mixture was placed at the bottom of an ultracentrifuge tube, and a discontinuous gradient was formed by overlaying with the 30% sucrose and 5% sucrose solutions.

Subsequently, the starch suspension was layered on the top of a 5 ml cushion consisting of 90% (v/v) Percoll (GE Healthcare Bio-Sciences AB, Uppsala, Sweden) and 10% (v/v) extraction buffer, the mixture was centrifuged for 15 min at 400 g.

After the addition of 10 μM ATP (in assay buffer), the mixture was incubated for 1 h at room temperature, followed by the addition of 20 μl of IMAP binding solution and a further 2 h of incubation at room temperature in the dark.

After addition of magnesium sulphate, sodium chloride and citrate buffer, the mixture is shaken intensively and centrifuged for phase separation.

Purified H3.5, H3T, or H3.3 was combined with H4 at a 1 1 molar ratio in unfolding buffer, and the mixture was dialyzed against refolding buffer, followed by stepwise salt-dialysis with refolding buffer containing 1, 0.5, and 0.1 M NaCl.

Purified H2A and H2B were mixed at a 1 1 molar ratio in unfolding buffer, and the mixture was dialyzed against refolding buffer.

The dried pellet was washed with 10 ml of 10 mM phosphate buffer (pH 6.8) again, re-suspended with 25 ml of the same buffer and the mixture was autoclaved for 20 min at 120°C.

One μg of each labeled cRNA was fragmented by adding 5 μl 10 × blocking agent and 1 μl of 25 × fragmentation buffer, then the mixture was heated at 60°C for 30 minutes; finally, 25 μl 2 × GE hybridization buffer was added to dilute the labeled cRNA.