Sentence examples for buffer the membrane was from inspiring English sources

After further washes in TBST wash buffer, the membrane was incubated with the primary antibody (Lifespan bioscience, Seattle, WA) at room temperature for two hours.

After incubating the membrane in the blocking buffer, the membrane was incubated with heat-shock protein 70 (HSP70) monoclonal antibodies (Santa Cruz Biotechnology, Santa Cruz, CA).

After several washes with washing buffer, the membrane was transferred to DAB assay reagents (Catalog No. P0203, Beyotime, China) for chromogenic detection.

After 3 times washes with TBST buffer, the membrane was incubated with anti-mouse-HRP and goat anti-rabbit-HRP for 30 minuates, respectively.

After washing with stripping buffer, the membrane was reprobed with GAPDH (1 : 5000, Kangchen, China), using ultra enhanced chemiluminescence western blotting detection reagents.

After removal from the incubation buffer, the membrane was washed extensively and incubated with a horseradish peroxidase-conjugated goat anti-rabbit secondary antibody (Merck Millipore) at room temperature, for 1 hour.

Unbound antibodies were removed by 2 min washing in detection buffer and the membrane was evenly covered with 1 ml of CSPD diluted 1/100 v/v in detection buffer.

After thorough washing with buffer A, the membrane was probed for caspase-9.

The RNA was then transferred to a nylon membrane (Ambion) in the same buffer and the membrane was baked for 30 min at 80°C.

After prehybridization, the purified labeled probe was added to the prehybridization buffer and the membrane was hybridized at 42°C for 12 – 18 h.

After stripping the membrane with Restore Plus Western Blot Stripping Buffer (Thermo Scientific), the membrane was blocked again and immunoblotted with anti-Erk1/2 antibody (Cell Signaling).