Sentence examples for buffer the material was from inspiring English sources

After rinsing in 0.1 M phosphate buffer, the material was dehydrated in a graded alcohol series and acetone, and embedded in Epon 812 epoxy resin.

After washing in 0.1 M phosphate buffer (pH 7.4), the material was fixed in 1% OsO4/0.1 M phosphate buffer (pH 7.3) at 3 °C for 1½ hours and washed again in 0.1 M phosphate buffer (pH 7.4).

Thereafter, 25 μl of dilution buffer was added and the material was ready for use in the qPCR.

After adding 400 µl RLT buffer (Qiagen, Hilden, Germany), the material was homogenized using a TissueLyser bead mill (Qiagen) and extracted using the BioSprint DNA Plant Kit and the BioSprint 96 workstation from Qiagen.

After that the preparations were washed in phosphate buffer for 20 min, and then the material was dehydrated in a rising series of acetone concentrations (30, 50, 60, 70, 80, 90 and 100%).

The material was dissolved in phosphate buffered saline (PBS) aqueous solution and ethanol PBS mixtures.

Next, the material was washed in buffer and postfixed in 1% osmium tetroxide for 1 h at 4°C.

To enable an ultrastructural analysis, the material was fixed in 4% buffered paraformaldehyde, postfixed in 1% OsO4, dehydrated with alcohol in increasing concentrations, and embedded in Epon-Araldite.

Measurements of moisture buffer value revealed that the material is classified as good or excellent depending on the percentage of fiber.

After three washes of the beads in lysis buffer the immunoprecipitated material was resolved by 12% SDS-PAGE and visualized by autoradiography.

After washing with equilibrating buffer, the adsorbed material was eluted with 0.1 M d-mannose in equilibrating buffer.