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Mixed with the fragmentation buffer, the mRNA was fragmented into short fragments (about 200 bp).
By mixing with fragmentation buffer, the mRNA was then broken into short fragments.
After mixing the fragmentation buffer, the mRNA was fragmented into short fragments.
After adding the fragmentation buffer, the mRNA was interrupted to short fragments (about 200 bp).
After adding fragmentation buffer, the mRNA was fragmented into short sequences (about 200 bp), first-strand cDNA was synthesized using random hexamer-primers and the mRNA fragments as templates, and then second strands were synthesized.
Similar(55)
Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments.
The Dynabeads were washed in washing buffer then the mRNA was eluted in TE buffer.
The mRNA was mixed with the fragmentation buffer and fragmented into short fragments.
The mRNA was eluted in 25 μl of elution buffer and stored at −80°C until cDNA synthesis could be performed.
The mRNA was broken into short segments by adding fragmentation buffer.
Followed by purification, the mRNA was fragmented into smaller pieces by the fragmentation buffer (Ambion).
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