Sentence examples for buffer the lysates were from inspiring English sources

After the cells were lysed by a nucleus lysis buffer, the lysates were sonicated.

Cells were then lysed using ice-cold lysis buffer; the lysates were further sonicated on ice.

Cells were directly lysed in RIPA buffer, the lysates were clarified by centrifugation and RhoA-GTP or Rac-GTP were quantified.

The cells were lysed in 0.3 0.75 ml ice-cold lysis buffer, the lysates were clarified by centrifugation at 4°C for 15 min at 26000  g and supernatants were frozen in liquid nitrogen.

To examine the interaction of optineurin with itself, RGC5 cells or those transfected with pTarget-FLAG-OPTN were lysed in IP buffer and the lysates were subjected to native blue gel electrophoresis.

In brief, after fractionation of cells into the cytoplasmic and nuclear fractions, the nuclear fraction was incubated with RIPA buffer and the lysates were subjected to SDS-PAGE electrophoresis using pre-casted gels from Nupage Bis-Tris (4 12%) gel systems (Invitrogen).

Cells co-expressing HA-hDAT and His-hDATΔEX6, or those co-expressing HA-mGLT1 and His-hDATΔEX6 were homogenized with RIPA buffer, and the lysates were subjected to immunoprecipitation overnight at 4°C with 0.5 µg of anti-His antibody, followed by 1 h of incubation with a 50% slurry of protein-A Sepharose beads (GE Healthcare Biosciences).

Cells were lysed in the lysis buffer and the lysates were centrifuged at 10,000 g for 10 min at 4°C.

Approximately 2 × 10 cells were lysed in lysis buffer and the lysates were centrifuged at 14,000 rpm for 15 minutes.

Cells were lysed in RIPA buffer, and the lysates were analyzed using a standard western blot procedure.

For Western blot analysis, tissues and cells were lysed by lysis buffer, and the lysates were collected.