Sentence examples for buffer the grids were from inspiring English sources

After washing with buffer, the grids were stained with 2% (w/v) uranyl acetate for 1 min at RT.

After washing in this buffer, the grids were incubated for 1 h with a rabbit anti-mouse antibody coupled to 10 nm gold particles diluted at 1 : 20 in incubation buffer.

After washing with buffer A, the grids were incubated with goat anti-rat secondary antibody labeled with 10-nm colloidal gold particles (BB International, UK) for 4 h at 35°C for the LM10 antibody (1 20 dilution in buffer A).

After washing in buffer and distilled water, the grids were stained for 45 min with 5% uranyl acetate and for 5 min with lead citrate and observed in a JEOL 1200EXII transmission electron microscope operated at 80kVV.

After 3 washes with wash buffer, the grid was incubated for 1 hr with 1∶10 diluted gold labeled Protein G secondary antibody (Aurion, The Netherlands).

Finally, the grids were washed by buffer A, followed by distilled water.

Following our previously published protocol (Sindelar and Downing, 2007), most of the initially applied buffer on the grids was wicked away by touching the grid edgewise with a piece of filter paper (Whatman).

Grids were immunolabeled in a two step method according to the following procedure; the grids were conditioned in phosphate buffered saline (PBS) for 5 min ×3 at room temperature (RT), followed by the blocking of non-specific labeling for 30 min at RT using 5% non-fat dry milk in PBS.

1.5 μl sample was applied to the carbon film, mixed thoroughly with excess of embedding buffer and incubated for 1 min. The grids were blotted and rapidly frozen in liquid nitrogen.

After suspension of the grids in buffer A (pH 8.2 Tris-buffered saline containing 1% bovine serum albumin) for 30 min at room temperature, the grids were incubated with LM10 (1:20 dilution in buffer A) for 2 days at 4°C.

After 6 washing steps, samples on the grids were fixed using virus buffer containing 1 % glutaraldehyde.