Sentence examples for buffer the grid was from inspiring English sources

After 3 washes with wash buffer, the grid was incubated for 1 hr with 1∶10 diluted gold labeled Protein G secondary antibody (Aurion, The Netherlands).

After washing with buffer, the grids were stained with 2% (w/v) uranyl acetate for 1 min at RT.

After washing in this buffer, the grids were incubated for 1 h with a rabbit anti-mouse antibody coupled to 10 nm gold particles diluted at 1 : 20 in incubation buffer.

The grid was washed immediately with 3 µl Buffer A, then blotted from the side.

The grid was then washed twice with 20 mM Tris, pH 7.4 buffer containing 30 mM imidazole and 150 mM NaCl.

The grid was then incubated with N.meningitidis PilQ monoclonal antibody [46] diluted 1∶1 in wash buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl) for 1 hr.

The grid was the great leveler.

Utilities have started to use batteries to buffer the grid, but they're not in wide use because of the cost.

Following our previously published protocol (Sindelar and Downing, 2007), most of the initially applied buffer on the grids was wicked away by touching the grid edgewise with a piece of filter paper (Whatman).

3. Excess fluid is drained off on to a piece of filter-paper and the grid is washed by adding a drop of phosphate buffer and redraining.

After washing with buffer A, the grids were incubated with goat anti-rat secondary antibody labeled with 10-nm colloidal gold particles (BB International, UK) for 4 h at 35°C for the LM10 antibody (1 20 dilution in buffer A).