Sentence examples for buffer the gel was from inspiring English sources

Following separation of the proteins on 4 12% Bis-Tris Novex gel (Invitrogen) in MOPS buffer, the gel was stained with Colloidal Blue (Sigma).

PCR was followed by electrophoresis in 15% polyacrylamide gel in a Tris/borate/EDTA buffer; the gel was stained with silver nitrate and photographed.

After migration using SDS-glycine buffer, the gel was incubated in 250 mM Tris-HCl, pH 8.5, for 10 minutes before incubation in the staining buffer (0.5 mg/ml hypoxanthine, 0.5 mg/ml thiazolyl blue tetrazolium bromide and 50 μg/ml phenazine methosulfate).

Excess ligand was eluted with 20 ml of coupling buffer and the gel was incubated in 0.1 M Tris-HCl buffer pH 8.0 for 2 hours at room temperature.

Excess mAb was removed by washing five times with 1.0 mL of coupling buffer, and the gel was allowed to stand in 4.0 mL of 0.1 M Tris-HCl buffer (pH 8.0) for 2 h at room temperature.

The lower chamber was then replenished with fresh buffer and the gel was run for an additional 45 minutes.

It was then replaced with fresh developing buffer and the gel was incubated at 37°C for 16 h.

After washing with 1 × MMP wash buffer for 15 min, the gel was incubated in 1 × MMP reaction buffer at 37℃ for 48 hr.

After equilibration with the clotting buffer the theoretical pH of the gel was 7.6 and osmolarity 320 mOsM.

To avoid the acidic quenching effect on PQDs (the destaining solution contains acetic acid, based on the anterior results), after running with SDS buffer for 90 min, the gel was imaged on the Tanon 2500 gel imaging system with UV light (365 nm) in advance.

The new Tris buffer was replaced and the gel was incubated for 4 hrs at 37°C to allow caseinolysis occur.