Sentence examples for buffer the enzyme was from inspiring English sources

After washing the column with standard buffer, the enzyme was eluted with a linear 0 to 0.5 M NaCl gradient in the same buffer.

The supernatant was put on a DEAE-cellulose column (3.0 × 14.0 cm) equilibrated with buffer A. After washing the column with the same buffer, the enzyme was eluted with buffer A containing 0.05 M NaCl.

The enzyme solution was then applied to a Mono Q (Pharmacia, Uppsala, Sweden) column (5.0 × 50 mm) equilibrated with buffer A. After the column was washed with the same buffer, the enzyme was eluted with a linear gradient of buffer A to buffer A containing 0.2 M NaCl.

The cell extract was diluted 3-fold with a buffer containing 20 mM Tris HCl, pH 7.6 (buffer A) and loaded onto a HiTrap Q HP column that had been previously equilibrated in buffer A. After the column was washed with five column volumes of buffer A, the enzyme was eluted with a linear gradient of 0 1 M NaCl in buffer A. Fractions with the enzyme eluted from 0.2 to 0.75 M NaCl.

The column was washed with 20 ml of resuspension buffer and the enzyme was eluted with 50 mM KPi buffer pH 8.0 (500 mM imidazol, 500 mM NaCl and 10% glycerol).

The column with 5 ml agarose-p-aminophenyl-β-D-thiogalactoside (Sigma Chemical, USA) was equilibrated with 50 mM phosphate buffer, and the enzyme was eluted with 0.1 M borate buffer, pH 10 (9). 1 ml aliquots were collected at a flow rate of 100 ml/min and pH was neutralized to avoid denaturation.

After the column was washed with 240 mL buffer A, the enzyme was eluted with 0.3 M NaCl in buffer A. The enzyme solution was then applied to a heparin-Sepharose column (1.6 cm × 15 cm), which was preequilibrated with buffer A containing 0.2 M NaCl.

After the column was washed with five column volumes of buffer B, the enzyme was eluted with a linear gradient of 0 0.5 M imidazole in buffer B. The peak of enzyme activity eluted at an imidazole concentration range of 0.06 0.27 M.

The column was washed with the same buffer and the enzyme was eluted with a linear gradient of 0 0.5 M NaCl in HEPES buffer at a flow rate of 0.5 mL/min [50].

The column was then washed with the same supplemented buffer, and the enzyme was eluted with a linear 1.0 to 0 M (NH4 2SO4 gradient in the same buffer.

The solid residue, mainly constituted by cell walls and vascular fibers, was washed with the same buffer and the enzyme was finally eluted from the solid residue with 500 mL of 0.1 M sodium phosphate buffer (pH 7) and, then, centrifuged.