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After 3 washes of 15 minutes in 0.1 M phosphate buffer, the ears were decalcified in 4.3% EDTA in 0.1 M phosphate buffer pH 7.3 for 48 hours after which they were dissected to expose the organ of Corti.
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The ears were cleaned with water (25 ± 0.5°C), and the ears with injuries were discarded.
The ears are fragile instruments.
The ears are $1.50 each.
The ears are small.
After three quick washes with 0.1 M sodium cacodylate buffer, the inner ears were post-fixed in 1% osmium tetroxide in 0.1 M sodium cacodylate buffer for 1 h at room temperature.
Tissue samples from the ear were dissolved in a buffer containing (50 mM KCl, 10 mM Tris-HCl, pH 8.3, 2 mM MgCl2, 0.1 mg/ml gelatin, 0.45% NP-40, 0.45% Tween-20, 0.5 mg/ml proteinase K) at 55°C for overnight.
For gross morphology on plastic sections, the ear was removed from the temporal bone and the inner ears were dissected in phosphate buffered saline (PBS), perfused with 4% paraformaldehyde and kept in the same fixative at room temperature for at least 12 hours.
The ear is wonderful.
The ear is the common site for keloid formation especially in women after ear piercing.
The ear is irrigated.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com