Sentence examples for buffer the concentration of from inspiring English sources
An ability of one Ste50 to simultaneously bring two molecules of Ste11 to the site of active Ste20 on the cortex may significantly enhance the efficiency of Ste11 phosphorylation by Ste20, while the large Ste50 homo-oligomers may be a dynamic reservoir for Ste50 that could buffer the concentration of Ste50 monomers available for interaction with Ste11.
This indicates that homosubstituted imines 3 and 4 are acting as "sinks" that modulate and buffer the concentration of imines 1 and 2 by preferential incorporation of newly added amine.
Mrs1 was expressed and purified as described [18] except that Mops was used as the buffer, the concentration of DTT was 5 mM, and glycerol was 10% (v/v).
Sperm samples from several different individuals were later pooled and diluted 5-fold in phosphate buffer; the concentration of this dilution was determined to be 915 μg/ml.
Following sample preparation and reconstitution in stacking buffer, the concentration of each steroid was 50 nM when 100%% recovery was achieved.
The variables were the percentage of blocking buffer, the concentration of the recombinant proteins used, the dilution of the antibody detecting the antigens (prM and E), and the secondary antibody conjugated with HRP.
Especially, components of protein extraction buffer (PEB) are well investigated, such as the pH of buffer, the concentrations of SDS in buffer with or without DTT [ 11- 17].
Due to the applied extraction procedure (2.5 g of oil with 2.5 mL of extraction buffer), the concentrations of hazelnut proteins in the oil (μg/g) are similar to the concentrations in the extraction buffer (μg/mL).
To avoid the effects of blood buffer capacity, the concentration of working buffer in the measurement cell should therefore be not less than 5 mM.
γ-PGA were dissolved in PBS buffer at the concentration of 0.025, 0.1, 0.25%w/v.
Then, the sample was incubated with BSA in PBS buffer at the concentration of 1 mg/ml for 20 h at room temperature.
