Sentence examples for buffer the cells were from inspiring English sources

After washing with phosphate-buffered saline (PBS) buffer, the cells were first treated for 5 min with 0.25% Trypsin-EDTA (Biochrom) at 37°C, followed by 15 min treatment with dispase/DNaseI solution (20 U/ml dispase (Worthington) and 100 U/ml DNase I (Sigma) in Quantum 286 medium) at 37°C.

After washing with FACS buffer, the cells were analyzed by a FACSCalibur (BD Bioscience, Franklin Lakes, NJ, USA) and Flowjo software (Tree star, Ashland, OR, USA)66.

After additional washes in buffer, the cells were dehydrated and embedded with Epon 812 (Shell Chemical, UK), followed by polymerization.

After washing with the phosphate buffer, the cells were dehydrated in a series of ethanol solutions of 30%, 50 %, 70 %, 95 and 100% for 50 min, and then embedded with Epon 8100%Shell Chemical, London, UK) for polymerization.

Cells were fixed in precooled 70%% ethanol (diluted with PBS) for 2 h and then washed with ice-cold PBS twice; after it was suspended with budding buffer, the cells were treated with RNaseA and stained with propidium iodide for 30 min at room temperature.

Following two washes with washing buffer the cells were resuspended in 1 mL of washing buffer.

After washing with cold phosphate buffered saline, the cells were lysed using sonication.

After washing with SAP-buffer, the cells were incubated with a secondary goat anti-mouse Fluorescein Isothiocyanate-labeled antibody (DakoCytomation, Glostrup, Denmark) or sheep anti-rabbit Phycoerythrin-labeled antibody (Serotec, Oxford, UK) in the dark for 40 min at 4°C.

Then, the samples were washed with 2 mL permeabilization buffer, and the cells were suspended in Macs buffer for flow cytometric analysis [15].

Following induction for 44 hours, the dishes were rinsed, using ice-cold PBS buffer and the cells were then harvested in buffer A. All further steps were carried out at 0° to +4°C.

Excess Fura 2-AM was removed by washing twice with CM buffer, and the cells were finally resuspended in CM buffer at 0.5×106 cells/ml.