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During the run and in order to avoid sample carry-over into the electrophoresis buffer, the capillary was dipped twice in distilled water and washing buffer (same electrophoresis buffer that set in other tubes).
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The capillary was rinsed with buffer SHs directly before the CE-LIF runs or with buffer SHval before runs of depolarized controls.
After injection, the inlet of the capillary was rinsed twice in SH buffer and switched to the vial of running buffer (buffer SHs or SHval for depolarized controls).
The capillary was pretreated with preconditioning buffer including 25 mM ammonium acetate and 75 mM sodium phosphate at pH 8.5.
For FM and CM the capillary was filled with analyte free buffer prior to immersion of the sample.
Between each run, the capillary was flushed with distilled water (1 min) and electrolyte buffer (3 min).
The capillary was rinsed with 0.15 M NaOH, water, and TGK buffer between each run.
The capillary was rinsed with 1 M NaOH followed by running buffer to prevent carryover between runs.
The capillary was rinsed with 1 M sodium hydroxide followed by running buffer to prevent carryovers.
For each run, the capillary was conditioned by rinsing with 0.1 M NaOH, water and running buffer for 3 min, respectively.
The capillary was rinsed with 0.1 M NaOH for 5 min and running buffer for 5 min between runs.
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