Sentence examples for buffer the biotin was from inspiring English sources

Following one wash with ice-cold biotinylation buffer the biotin was quenched with two washes of quenching buffer (phosphate buffered saline supplemented with 10 mM Tris, 100 mM glycine, pH 8.0).

After washing with Tris buffered saline (TBS) with tween, the endogenous biotin was suppressed by sequential incubations with 0,1 % avidin and 0,01 % biotin (Dako, Glostrup, Denmark) for 10 min each at room temperature.

The amount of biotin was determined using the following equation: V biotin = 1, 000 × m PLGA M W biotin M W PLGA M biotin C biotin Open image in new window (2).

RNA in the supernatant was ethanol precipitated and resuspended in buffer A. Maleimido-biotin was added at a final concentration of 100 µM, incubated at room temperature for 4 hr, and quenched with an excess of 2-mercaptoethanol.

Conjugate NIT82B (0.6 mg) was dissolved in in PBS buffer and NHS-biotin was dissolved in water (17 µl; 10 mM (0.88 mg biotin in 200 µl water)).

After antigen retrieval using a citrate buffer in a pressure cooker, endogenous biotin was blocked.

Immediately after the carboxylation, the carboxylated biotin is attached to the biotin carboxyl carrier protein, which is encoded by accB, resulting in the synthesis of carboxy-biotin.

50 µM biotin was added to the medium for 24 hr prior to lysing cells under denaturing conditions (M-lysis buffer; Roche).

Free biotin was quenched using Tris buffer (TBS) with 5% FBS, samples were washed twice in TBS, followed by lysis as above.

After antigen retrieval in heated Tris-EDTA-Tween buffer, endogenous peroxidase and biotin were blocked.

Shortly after intravenous biotin injection, the biotin label was found to be highly uniform (Fig. 1A).