Sentence examples for buffer the beads were from inspiring English sources

After extensively washing four times with the lysis buffer, the beads were loaded onto a column and rinsed twice with phosphate-buffered saline.

After washing in 70% ethanol, incubation in denaturing buffer and flushing with wash buffer, the beads were then released into a 96-well plate containing annealing buffer and the specific sequencing primer.

After washing the beads 3 4 times in the same buffer, the beads were resuspended in SDS-PAGE sample buffer.

After three washes with the buffer, the beads were boiled in SDS loading buffer at 95oC for 5 min before electrophoresis.

After washing three times with IP buffer, the beads were treated with proteinase K (2 U; Invitrogen, Carlsbad, CA) for 3 h at 50°C.

After washing five times in lysis buffer, the beads were boiled in Laemmli buffer, the proteins were separated by SDS-PAGE, followed by immunoblotting as described previously [69].

After several washing steps with different buffers, the beads were collected and resuspended in 0.1 ml of 50 mM Tris-HCl, pH 7.0; 0.5 mM EDTA; 10 mM DTT and 1% SDS before incubated at 70 °C for 45 min to reverse crosslinking.

After washing with buffer-1 (1×) and buffer-2 (2×), the beads were resuspended in loading buffer, boiled under reducing conditions and subjected to western blot analysis.

After being washed with pull-down buffer twice, the beads were resuspended in sample buffer and subjected to Western blot analysis.

Samples were washed four times with lysis buffer before the beads were resuspended in SDS sample buffer and boiled for 5 min. Western analysis was performed using standard protocols and the following antibodies: EGFR (1∶1000, Santa Cruz), ErbB2 (1∶1000, Abcam), ErbB3 (1∶1000, Santa Cruz), ErbB4 (1∶1000, Santa Cruz); flag (1∶1000 M2, Sigma), and actin (1∶8000, Abcam).

The immunoprecipitates were washed three times with 10 ml of lysis buffer containing 0.5 M NaCl and twice with 10 ml of buffer A. The beads were resuspended in a total volume of 30 μl of LDS sample buffer (Invitrogen).