Sentence examples for buffer the aortas were from inspiring English sources

After washing with Krebs buffer, the aortas were carefully cut longitudinally and mounted en face (face down) on slides and then covered with cover slip for endothelial layer imaging.

After washing with phosphate buffered saline (PBS), the aortas were carefully cut longitudinally and mounted en face (face down) on slides and then covered with cover slip for endothelial layer imaging.

For electron microscopic observation, thoracic aortas were cut open longitudinally, rinsed with ice-cold 0.1 mol/L phosphate buffer (pH 7.2), and fixed with 3% glutaraldehyde for 2 h After being washed with 0.1 mol/L phosphate buffer for 3 times, the aortas were fixed with osmium tetroxide and dehydrated in ethanol.

The aortas were then washed twice with phosphate buffered saline (PBS) and fixed for 10 min with 10% formalin solution, followed by 60% isopropanol for 1 min.

The aortas were embedded in paraffin blocks.

The beating hearts were quickly removed and arrested in the ice-cold Krebs–Henseleit perfusion buffer, and the aorta was cannulated for perfusion in recirculating retrograde Langendorff mode at a constant 85 mmHg pressure and 37°C temperature.

The aortae were postfixed in 4% buffered formalin for 24 48 hours.

If the aorta were ruptured the victim would exsanguinate.

The aorta was homogenized in RIPA buffer with Proteinase Inhibitor Cocktail (P8430, Sigma) and Phosphatase Inhibitor Cocktail II (P5726, Sigma) to inhibit tyrosine protein phosphatases.

The aorta was cannulated under the buffer surface using a 21-gauge custom-made cannula, and was attached to the cannula needle using a micro aneurysm clip (Harvard Apparatus, Edenbridge UK).

The aorta was cut into rings about 5 mm long in Krebs buffer which was constantly gassed with 95%O2+5% 5% CO2 at 37 ± 0.5°C.