Sentence examples for buffer systems were used from inspiring English sources

Various buffer systems were used: NEBuffer 3, pH 7.9, NEBuffer EcoRI, pH 7.5 (both from New England Biolabs), PGM assay buffer, pH 7.0 (see above), and 1 M diethanolamine, 1 mM MgCl2, pH 9.75.

Two different buffer systems were used: Tricine (Fig. 10C) and Laemmli-urea (Fig. 10D).

The gene products of a total of 21 enzyme-coding loci were assayed for electrophoretic variability, the loci scored are given in Additional file 2. Two electrophoretic buffer systems were used.

The following buffer systems were used (50 mM): sodium acetate (pH 5.0), sodium citrate (pH 6.0), sodium phosphate (pH 7.0 and pH 8.0) and sodium carbonate (pH 9.0 and pH 10.0).

A pre-cast bis-tris gel (Invitrogen, Carlsbad/CA, USA) and a MOPS buffer system were used and standard Western blotting protocols were followed.

The same buffer system was used for both buffers and the total buffer volume was kept constant.

Comparing the two buffer systems, we found that 50% more proteins can be extracted from FFPE blocks that were stored for 20 years when the new buffer system is used.

For assembly, a phosphate buffer system was used, which enables the dynamic formation of authentic filaments from soluble tetramers by addition of salt to increase the ionic strength to physiological values [16].

SDS/PAGE was performed essentially as described by Blattler et al. [ 20] except that the Tris/glycine buffer system was used [ 4].

Tricine SDS-PAGE in a discontinuous gel and buffer system was used to estimate the molecular mass of the proteins, under reducing and nonreducing conditions [ 15].

An overlapping 100 mM buffer system was used (pyridine-HCl at pH 4.5, 5.0 and 5.5, and Bis-Tris HCl at pH 5.5, 6.0, 6.5 and 7.0) to determine the pH response of GAD activity.